Alu elements are short interspersed, repeated dna elements distributed through out the primate genomes. Laboratory exercise dna extraction techniques for use in education received for publication, march 29, 2009 r. Dna extraction lab 1 cheek cell dna extraction capture your genes in a bottle. Fluorescent cell imaging activities for your classroom biorad zoe. Cells come in a variety of shapes and sizes, and cells perform different functions. Laboratory exercise dna extraction techniques for use in. The main cell structures are easy to see when a stain is used and the slide is viewed with the microscope. Students can easily view their own cells as an example of eukaryote cells and cell theory. This one period lab is a great way to introduce microscope skills or view animal cells using the students own cheek cells.
This dye is toxic when ingested and it causes irritation when in contact with the skin and eyes. Extracting human dna in the classroom buccal cheek cells can be harvested painlessly and in sufficient quantity to visualise dna extracted in a simple 4step protocol we will be carrying out an optimised dna. Onion and cheek cell lab microbiology by lillian c. Move your fingers down and to the right to place the cover slip over the cheek cell. Learn how to extract dna from cells and describe the purpose of the. In this experiment you would use dna isolated from your cheek cells see above as template to amplify a short region of dna within the plasmogen activator gene tpa containing a short 300bp insertion called an alu element.
Human cheek cells experiment from microscopes for schools. Dna isolation using human cheek cells introduction this experiment shows how to isolate human dna from the cells of your cheek. Study 50 terms dna cheek cells lab flashcards quizlet. In this lab, you will prepare and observe cells from both a plant onion and an animal your own cheek. List 2 organelles that were not visible but should have been in the.
The alu element found within an intron of the tpa gene is dimorphic. If i look at my cheek cell scraping under a microscope and put iodine on it, then i will see the different parts of the cell including the nucleus, cytoplasm, and cell membrane. Ncert class 9 science lab manual slide of onion peel and cheek cells aim to prepare stained temporary mounts of onion peel and human cheek cells and to record observations and draw their labelled diagrams. The virtual microscope is a little more complicated than the microscope we used in the lab, but it will not be difficult to use. The cells that line the inside of your cheek are called epithelial cells. Put a drop of water in the center of a glass slide. To view cheek cells, gently scrape the inside lining of your cheek with a toothpick. Manipulation of apparatus involves manual dexterity and efficiency e. In class, we obtained cheek cells by scraping the inside of the mouth with a toothpick and then rubbing the toothpick on a drop of water with blue. This laboratory course is designed to familiarize you with techniques used in cell biology. Turn the microscope nose piece so that the 4x objective is over the light. Introduction to cells, cheek cell and onion cell lab, lesson powerpoint 1.
Learn how to extract dna from cells and describe the. They are constantly being soughed off like the cells of the outer skin. What structure can be seen with the aid of the iodine stain. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Compound microscope describe the location and function of the following parts of the microscope. Dna carries genetic information that is inherited, or passed down from parents to offspring.
You will need to provide slides of cheek cells, onion cells, elodea, and volvox. Please complete your lab report on separate lined paper and include each. I drank celery juice for 7 days and this is what happened no juicer required. Position a cover slip at a 45 degree angle just inside the left edge of the solution. Place your thumb over the end of your test tube and. If you missed the microscope lab we did in class, you will need to make it up by using a virtual microscope which can be accessed on the internet. In this lab you will look at two types of cells, a human cheek cell and an onion cell and see how they are similar and how they are different.
Dna is used every day by scientists and lawyers to help in criminal investigation, paternity suits, cloning, etc. The cells seen are squamous epithelial cells from the outer epithelial layer of the mouth. A cell membrane that is semipermeable surrounds the cytoplasm. Add one drop of methylene blue onto the water and cell solution to stain the cheek cells for observation. Keeping in mind that the mouth is the first site of chemical digestion in a human. Choose one of the cell labs onion cell, cheek cell that you completed this week and report out your findings using the following lab report format.
They draw and label what they observe under high power. A series of reagents will be used to separate the dna from the cells. Place a small amount of onion tissue on the glass slide. Read pdf elodea cheek cell lab answers plasmolysis in elodea plant cells science netlinks purpose the purpose of this lab is to learn how to prepare a wet mound, to learn proper staining techniques and to examine human cheek cells and onion skin cells. Microscope and the cell lab report purpose of the microscope describe the correct way to handle a microscope. Onion peel the cells are very clearly visible as compartments with. Methylene blue stains negatively charged molecules in the cell, including dna and rna.
Ocular condenser stage course adjustment knob describe how total magnification is calculated. Used inoculating loopsswabs and slides with cheek cells should be sterilised with. Stepbystep video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells. Deoxyribonucleic acid dna is a molecule present in all living things, including bacteria, plants, and animals. Bring the cup containing the water and your cheek cells into the lab and pour a few ml into the larger test tube. Combining two lenses increases the magnification but decreases the. To observe and identify major animal cell structures and to relate the structure of the cell to its function. Unlike plant cells, the cytoplasm in an animal cell is denser, granular and occupies a larger space. By the end of the semester you should be familiar with. A neat lab sheet introduces cell biologists to the general structure of both animal and plant cells. Focus your cell phone microscope on your cheek cells and take a cellfie.
Take a small drink of water from a nearby drinking fountain and swirl it around in your mouth several times without swallowing and then spit the water into the cup. Pour the mixture into the blender and blend it for no more than 3 seconds. The light microscope used in the lab is not powerful enough to view other organelles in the cheek cell. Ncert class 9 science lab manual slide of onion peel and. Some scientists have speculated that the evolution of eucaryotic cells involved the merging of two. Take the gatorade from the cup into your mouth, and swish the water around vigorously for 60 seconds. This activity provides an opportunity to accomplish two tasks in our ap biology adventure.
In plant and animal cells, similarities and differences exist because of varied life functions. You can make a lab anywhere by using a cell phone and some household items. List 2 organelles that were not visible but should have been in the cheek cell. Onion tissue as well as cheek epithelial tissue provide excellent cells to study under the microscope.
How are cheek cells different from the plant cells you have studied. List 2 organelles that were not visible but could be found in cells if you had a microscope with a better magnification. Dna extraction from cheek cells the same general procedures, with little modification, for extracting dna can be used with a variety of plant and animal cells. The small blue dots are bacteria from our teeth and mouth. As in all animal cells, the cells of the human cheek do not possess a cell wall. Although cells may appear outwardly different, they resemble each other because they share common structures. Cheek cell dna extraction capture your genes in a bottle. The two scales both the micrometer is adjusted so that the zero of two scales merge or. Amplification of a specific dna sequence by polymerase. Using the provided pipet, slowly add 10 ml of the ice cold alcohol by letting it run gently run down the side of the bottle hold the bottle at an angle. This procedure teaches the properties of cells, cell.
Add a small drop of iodine stain directly to the tissue. Observing cells from a human cheek and bacteria under a compound microscope. Introduction to cells, cheek cell and onion cell lab. Virtual microscope lab cheek cells the biology corner. The cell lab student report page pre lab questions. As a result, some of these cells can easily and painlessly be. First of all, we will hopefully be able to see our own dna from. To find out what an animal cells look like under a microscope. Buccal cheek cells can be harvested painlessly and in sufficient.
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